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prime script 1st strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa prime script 1st strand cdna synthesis kit
    Prime Script 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11036 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prime+script+1st+strand+cdna+synthesis+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/10__1016_slash_j__biteb__2026__102870-131-17-24
    Average 99 stars, based on 11036 article reviews
    prime script 1st strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: JPI-547, a novel dual inhibitor of PARP1/2 and tankyrase is more effective than first-generation PARP inhibitors in preclinical BRCA1/2-mutated cancer models.
    Article Snippet: Quantitative real-time polymerase chain reaction (qRT-PCR) To study the mRNA expression levels, total RNA was extracted using TRIzol (cat# 15596018, Life Technologies, USA) according to the manufacturer's instructions. .. The cDNA synthesis of extracted the total RNA was performed with Takara Prime Script 1st Strand cDNA Synthesis Kit (cat# 6110 A, Takara Bio Inc., Japan) following the manufacturer’s instructions. .. PCR amplification was carried out with Power-up SYBR Green Master Mix (cat# A25741, ThermoFisher Scientific, USA) using an ABI Step One Real-time PCR System (Applied Biosystems, UK).

    Article Title: Tracking the clinico-microbiological profile and molecular characterization of dengue cases during the monsoon-season in Belagavi, Karnataka.
    Article Snippet: .. All NS1 antigen-positive samples were subjected to dengue serotyping using the DiA Sure Dengue Genotyping Kit (DiaSure Biotech, India). cDNA was synthesised using the Prime Script 1st Strand cDNA synthesis Kit (Takara Bio USA, Inc.), and RT PCR targeting the capsid pre-membrane (CprM) gene was carried out as described by Lanciotti et al [12]. .. Amplified PCR products were visualized and the target amplicon was purified using QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany).

    Article Title: Tracking the clinico-microbiological profile and molecular characterization of dengue cases during the monsoon-season in Belagavi, Karnataka
    Article Snippet: .. All NS1 antigen-positive samples were subjected to dengue serotyping using the DiA Sure Dengue Genotyping Kit (DiaSure Biotech, India). cDNA was synthesised using the Prime Script 1st Strand cDNA synthesis Kit (Takara Bio USA, Inc.), and RT PCR targeting the capsid pre-membrane (CprM) gene was carried out as described by Lanciotti et al [ ]. .. Amplified PCR products were visualized and the target amplicon was purified using QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany).

    Article Title: Valorization of the oleaginous fungus Mortierella alpina for sustainable aquafeed development: Physiological and microbiome responses in zebrafish (Danio rerio)
    Article Snippet: Microbial bioresources are emerging as sustainable alternatives to traditional aquaculture feed ingredients.. While microbial oils derived from oleaginous microorganisms have demonstrated beneficial effects in aquaculture nutrition, the functional role of whole-cell biomass remains insufficiently explored.. Therefore, in this study, dried biomass of the oleaginous fungus Mortierella alpina EI-9 was assessed for safety and physiological effects in juvenile and adult zebrafish (Danio rerio) over a 60-day feeding trial.

    Article Title: Protective effect of Lactococcus lactis NZ9000 secreting Bcl2 against Trichinella spiralis infection in mice.
    Article Snippet: .. The total RNA was isolated from jejunal tissue with Trizol Reagent (Invitrogen, USA), and then reverse transcribed into the first-strand cDNA using the Prime Script 1st Strand cDNA Synthesis Kit (Takara, Japan). ..

    Article Title: Harnessing plant-derived bioactive compounds as LOX-1 inhibitors in Helicobacter pylori-driven gastric inflammation.
    Article Snippet: Helicobacter pylori persistence and rising antibiotic resistance have intensified interest in host-directed adjunctive strategies.. Recent studies identify LOX-1 as a gastric epithelial receptor for H. pylori catalase and support its role in bacterial adhesion, making it a plausible host-directed target.. In the current study, BI-0115, a structurally defined selective LOX-1 inhibitor, was used for functional validation in gastric epithelial cells infected with two clinical H. pylori isolates.

    Article Title: High UBE2D1 expression is associated with poor prognosis and immunotherapy resistance in head and neck cancer
    Article Snippet: TRIzol reagent (Thermo Fisher Scientific Inc., USA) was used to extract total RNA, and Nanodrop One was employed to measure the RNA's quantity and quality. .. Complementary DNA (cDNA) synthesis was performed using the Prime Script 1st Strand cDNA Synthesis Kit (Takara Bio Inc., Tokyo, Japan). .. The SYBR Green qPCR Kit was utilized to carry out qPCR reactions in a Bio-Rad CFX Opus qPCR.

    Article Title: Protective effect of Lactococcus lactis NZ9000 secreting Bcl2 against Trichinella spiralis infection in mice
    Article Snippet: .. The total RNA was isolated from jejunal tissue with Trizol Reagent (Invitrogen, USA), and then reverse transcribed into the first-strand cDNA using the Prime Script 1st Strand cDNA Synthesis Kit (Takara, Japan). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Tracking the clinico-microbiological profile and molecular characterization of dengue cases during the monsoon-season in Belagavi, Karnataka.
    Article Snippet: .. All NS1 antigen-positive samples were subjected to dengue serotyping using the DiA Sure Dengue Genotyping Kit (DiaSure Biotech, India). cDNA was synthesised using the Prime Script 1st Strand cDNA synthesis Kit (Takara Bio USA, Inc.), and RT PCR targeting the capsid pre-membrane (CprM) gene was carried out as described by Lanciotti et al [12]. .. Amplified PCR products were visualized and the target amplicon was purified using QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany).

    Article Title: Tracking the clinico-microbiological profile and molecular characterization of dengue cases during the monsoon-season in Belagavi, Karnataka
    Article Snippet: .. All NS1 antigen-positive samples were subjected to dengue serotyping using the DiA Sure Dengue Genotyping Kit (DiaSure Biotech, India). cDNA was synthesised using the Prime Script 1st Strand cDNA synthesis Kit (Takara Bio USA, Inc.), and RT PCR targeting the capsid pre-membrane (CprM) gene was carried out as described by Lanciotti et al [ ]. .. Amplified PCR products were visualized and the target amplicon was purified using QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany).

    Reverse Transcription:

    Article Title: Valorization of the oleaginous fungus Mortierella alpina for sustainable aquafeed development: Physiological and microbiome responses in zebrafish (Danio rerio)
    Article Snippet: Microbial bioresources are emerging as sustainable alternatives to traditional aquaculture feed ingredients.. While microbial oils derived from oleaginous microorganisms have demonstrated beneficial effects in aquaculture nutrition, the functional role of whole-cell biomass remains insufficiently explored.. Therefore, in this study, dried biomass of the oleaginous fungus Mortierella alpina EI-9 was assessed for safety and physiological effects in juvenile and adult zebrafish (Danio rerio) over a 60-day feeding trial.

    Article Title: Protective effect of Lactococcus lactis NZ9000 secreting Bcl2 against Trichinella spiralis infection in mice.
    Article Snippet: .. The total RNA was isolated from jejunal tissue with Trizol Reagent (Invitrogen, USA), and then reverse transcribed into the first-strand cDNA using the Prime Script 1st Strand cDNA Synthesis Kit (Takara, Japan). ..

    Article Title: Protective effect of Lactococcus lactis NZ9000 secreting Bcl2 against Trichinella spiralis infection in mice
    Article Snippet: .. The total RNA was isolated from jejunal tissue with Trizol Reagent (Invitrogen, USA), and then reverse transcribed into the first-strand cDNA using the Prime Script 1st Strand cDNA Synthesis Kit (Takara, Japan). ..

    Isolation:

    Article Title: Protective effect of Lactococcus lactis NZ9000 secreting Bcl2 against Trichinella spiralis infection in mice.
    Article Snippet: .. The total RNA was isolated from jejunal tissue with Trizol Reagent (Invitrogen, USA), and then reverse transcribed into the first-strand cDNA using the Prime Script 1st Strand cDNA Synthesis Kit (Takara, Japan). ..

    Article Title: Protective effect of Lactococcus lactis NZ9000 secreting Bcl2 against Trichinella spiralis infection in mice
    Article Snippet: .. The total RNA was isolated from jejunal tissue with Trizol Reagent (Invitrogen, USA), and then reverse transcribed into the first-strand cDNA using the Prime Script 1st Strand cDNA Synthesis Kit (Takara, Japan). ..



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